Products Description
Ananas comosus (L.) Merr. belongs to the Bromeliaceae family and is one of the world's most commercially important tropical fruits, cultivated across Central and South America, Southeast Asia, and West Africa. While fresh pineapple is widely consumed, the nutraceutical industry's interest centers on its concentrated extract - a standardized powder derived from the fruit flesh, stem, or core that delivers a consistent, measurable dose of bioactive compounds.
Pineapple Extract Powder is produced by enzymatic or solvent-assisted extraction of fresh or dried pineapple material, followed by filtration, concentration, and spray-drying. The result is a light yellow to off-white fine powder with a characteristic mild fruity-sweet aroma, water-soluble, and stable under appropriate storage conditions.
At Joywin Natural, we supply pharmaceutical- and food-grade Pineapple Extract Powder standardized to defined bromelain activity (GDU/g or MCU/g), polyphenol content, and Vitamin C levels - with full traceability, cGMP manufacturing, and the documentation infrastructure that serious B2B buyers require. This page gives you the complete picture: the science, the specs, the sourcing, and the supply chain.
What makes pineapple extract commercially compelling isn't just one compound - it's the synergy between:
* Bromelain - a cysteine protease complex with anti-inflammatory, digestive, and immunomodulatory properties
* Polyphenols - including flavonoids and hydroxycinnamic acids with antioxidant activity
* Vitamin C - a well-established antioxidant and immune cofactor
* Organic acids - citric and malic acid contributing to metabolic and digestive support
"Bromelain exhibits a wide range of biological activities, including antioxidative, anti-inflammatory, immunomodulatory, antinociceptive, antiulcerative, and antihyperlipidemic effects." - Frontiers in Nutrition, 2026
Source & Raw Material Origin
The quality of pineapple extract is inseparable from the quality of the raw material - and that starts with geography and agricultural practice.
Our pineapple raw material is sourced from select cultivation regions in Guangdong and Hainan provinces, China, as well as contracted farms in Costa Rica and the Philippines - all recognized globally for producing high-Brix, enzyme-rich pineapple varieties. We work with farms that:
Cultivate MD-2 and Smooth Cayenne varieties, known for their high bromelain and polyphenol content
Practice GAP (Good Agricultural Practice) standards with documented pesticide management programs
Harvest at optimal maturity windows to maximize bromelain enzymatic activity - a critical factor often overlooked by lower-tier suppliers
Provide full batch traceability from farm lot to extraction
Stem vs. Fruit: What's the difference?
This is a question that comes up regularly in formulation discussions. The pineapple stem contains significantly higher bromelain concentrations than the fruit flesh - typically 2–3× higher enzymatic activity per gram of dry weight. For high-potency bromelain applications (digestive enzymes, sports recovery), stem-derived extract is the preferred source. For full-spectrum pineapple extract applications (antioxidant blends, functional foods, flavor-forward formulations), fruit-derived extract offers a more balanced bioactive profile with superior organoleptic properties. We offer both.

Key Bioactive Compounds & Ingredient Profile
Pineapple extract is not a single-molecule ingredient. Its functional value comes from a carefully preserved matrix of bioactives:
* Bromelain (Proteolytic Enzyme Complex)
The flagship bioactive. Bromelain is classified as a cysteine proteinase - it uses a cysteine thiol group in its active site to catalyze the breakdown of proteins into smaller peptides and amino acids. It is stable between pH 7–10 and maintains activity up to approximately 65°C, making it compatible with most supplement and food processing conditions.
Bromelain's activity is measured in GDU (Gelatin Digesting Units) or MCU (Milk Clotting Units) - not by weight alone. This is a critical point for formulators: two products both labeled "100mg bromelain" can have dramatically different biological activity depending on the GDU/g specification. Our extracts are standardized to defined activity levels, not just weight.
* Polyphenols & Flavonoids
Including ferulic acid, caffeic acid, and various flavonoid glycosides. These compounds contribute to the extract's antioxidant capacity, measured by DPPH and FRAP assays. Polyphenol content varies by variety and extraction method; our standard extract delivers ≥5% total polyphenols.
* Vitamin C (Ascorbic Acid)
Pineapple is a natural source of Vitamin C, and our whole-fruit extracts preserve a meaningful ascorbic acid content - relevant for immune support and antioxidant formulations. Standardized Vitamin C content is available upon request.
* Organic Acids
Citric acid, malic acid, and quinic acid contribute to the extract's digestive support properties and characteristic flavor profile - useful in functional beverage and food applications.
* Glycoproteins & Peroxidases
Co-extracted with bromelain, these compounds contribute to the extract's immunomodulatory and wound-healing properties observed in clinical research.
Product Specifications & COA Parameters
We offer multiple grades to match your formulation and regulatory requirements:
|
Parameter |
Standard Grade |
High-Activity Bromelain Grade |
Whole-Fruit Extract Grade |
Test Method |
|
Botanical Source |
Ananas comosus |
Ananas comosus (stem) |
Ananas comosus (fruit) |
- |
|
Bromelain Activity |
≥ 100 GDU/g |
≥ 2,400 GDU/g |
≥ 50 GDU/g |
FCC Method |
|
Total Polyphenols |
≥ 5% |
≥ 3% |
≥ 8% |
UV-Vis (Folin-Ciocalteu) |
|
Vitamin C |
≥ 1% |
- |
≥ 2% |
HPLC |
|
Appearance |
Light yellow fine powder |
Off-white to cream powder |
Light yellow fine powder |
Visual |
|
Particle Size |
80 mesh (95% pass) |
80 mesh (95% pass) |
80 mesh (95% pass) |
Sieve Analysis |
|
Moisture Content |
≤ 5% |
≤ 5% |
≤ 5% |
Loss on Drying |
|
Ash Content |
≤ 5% |
≤ 4% |
≤ 6% |
Gravimetric |
|
pH (1% solution) |
4.5–6.5 |
4.5–6.0 |
4.0–6.5 |
pH Meter |
|
Heavy Metals (Total) |
≤ 10 ppm |
≤ 10 ppm |
≤ 10 ppm |
ICP-MS |
|
Lead (Pb) |
≤ 0.5 ppm |
≤ 0.5 ppm |
≤ 0.5 ppm |
ICP-MS |
|
Arsenic (As) |
≤ 1.0 ppm |
≤ 1.0 ppm |
≤ 1.0 ppm |
ICP-MS |
|
Pesticide Residues |
EU 396/2005 compliant |
EU 396/2005 compliant |
EU 396/2005 compliant |
GC/LC-MS |
|
Total Plate Count |
≤ 10,000 CFU/g |
≤ 10,000 CFU/g |
≤ 10,000 CFU/g |
USP <61> |
|
Yeast & Mold |
≤ 1,000 CFU/g |
≤ 1,000 CFU/g |
≤ 1,000 CFU/g |
USP <62> |
|
E. coli |
Negative |
Negative |
Negative |
USP <62> |
|
Salmonella |
Negative / 25g |
Negative / 25g |
Negative / 25g |
USP <62> |
|
Shelf Life |
24 months |
24 months |
24 months |
- |
|
Storage |
≤ 25°C, dry, away from light |
≤ 25°C, dry, away from light |
≤ 25°C, dry, away from light |
- |
Full COA, MSDS, and third-party lab reports are available upon request. Custom specifications - including organic-certified grades, enteric-coated bromelain, or specific GDU/g targets - are available for qualified buyers.
Manufacturing Process
Enzyme stability is the central challenge in pineapple extract manufacturing. Bromelain is sensitive to heat, oxidation, and pH extremes - meaning every step of the production process must be engineered to preserve enzymatic activity while delivering a safe, stable, shelf-stable powder.
Step 1 - Raw Material Sourcing & Incoming QC
Fresh or dried pineapple material arrives with full farm documentation. Every incoming batch undergoes identity verification, moisture testing, initial bromelain activity screening, and pesticide residue testing before entering production.
Step 2 - Pre-Processing & Milling
Raw material is cleaned, sorted, and size-reduced under controlled temperature conditions (below 40°C) to prevent premature enzyme denaturation. For stem-derived extract, the fibrous core is separated and processed separately to maximize bromelain yield.
Step 3 - Aqueous Extraction
We use a low-temperature aqueous extraction process (typically 4–15°C) to solubilize bromelain and co-extract polyphenols and Vitamin C. Extraction pH is carefully controlled within the enzyme's stability window (pH 7–10). This cold extraction approach is a key differentiator - many lower-cost producers use elevated temperatures that damage enzymatic activity.
Step 4 - Clarification & Filtration
The crude extract undergoes centrifugation and multi-stage membrane filtration (ultrafiltration, 10–30 kDa cutoff) to remove insoluble fiber, cellular debris, and non-target compounds while retaining the target molecular weight fraction containing bromelain.
Step 5 - Concentration
Filtered extract is concentrated under vacuum evaporation at reduced temperature (below 50°C) to preserve enzymatic activity. Concentration is monitored in real time by activity assay.
Step 6 - Spray Drying Concentrated extract is spray-dried using food-grade maltodextrin or acacia gum as a carrier (or carrier-free for high-purity grades). Inlet temperature is optimized to balance moisture removal with enzyme preservation - typically 150–170°C inlet / 70–80°C outlet, with residence time minimized.
Step 7 - Standardization, Testing & Release
Finished powder is tested for bromelain activity (GDU/g), polyphenol content, Vitamin C, microbiological parameters, and heavy metals. Batches are blended to meet target specifications. Full release documentation is generated before shipment.
Quality Control & Certifications
Enzyme-based ingredients demand a higher standard of quality control than most botanical extracts - because activity, not just composition, must be verified and maintained through the supply chain.
Our QC infrastructure includes:
* In-house analytical laboratory with HPLC, GC-MS, ICP-MS, UV-Vis spectrophotometry, and enzymatic activity assay capability
* Bromelain activity testing per FCC (Food Chemicals Codex) method - the industry-standard protocol for GDU/g determination
* Third-party verification through accredited external labs (SGS, Eurofins, or equivalent) for every commercial batch
* Cold-chain monitoring during storage and shipping for high-activity enzyme grades
* Full batch traceability from raw material farm lot to finished goods shipment
* Stability testing program supporting 24-month shelf life claims under ICH Q1A conditions
Certifications held by Joywin Natural:
|
Certification |
Scope |
|
cGMP |
Manufacturing quality management |
|
ISO 9001 |
Quality management systems |
|
ISO 22000 |
Food safety management |
|
FSSC 22000 |
Food safety system certification |
|
BRC |
British Retail Consortium food safety |
|
Kosher |
Jewish dietary law compliance |
|
HALAL |
Islamic dietary law compliance |
|
ISO 14001 |
Environmental management |
All certificates are available for download or provided with quotation packages. We support customers through FDA registration, EU regulatory documentation, and Health Canada NPN dossier preparation upon request.
Health Benefits & Scientific Evidence
The following summarizes peer-reviewed research on Ananas comosus extract and bromelain. These statements reflect published scientific findings and are intended for formulation and R&D professionals - not as finished product health claims.
* Anti-Inflammatory & Analgesic Activity
This is the most clinically validated application for bromelain. The mechanism is well-characterized: bromelain inhibits NF-κB signaling (via suppression of IκBα degradation), downregulates MAPK pathways (ERK, JNK, p38), and reduces pro-inflammatory cytokines including TNF-α and IL-6. Multiple randomized clinical trials have demonstrated bromelain's efficacy in reducing post-surgical swelling, pain, and trismus - particularly in oral surgery contexts.
A 2024 three-arm randomized clinical study published in Nutrients found that pineapple extract and bromelain-based treatment significantly reduced edema, pain, and trismus following mandibular third molar surgery, with a favorable safety profile compared to NSAIDs.
* Digestive Enzyme Support
Bromelain's proteolytic activity makes it a natural fit for digestive enzyme formulations. It breaks down dietary proteins into peptides and amino acids, supporting protein digestion and nutrient absorption. Research published in Nutrients (Albert Einstein College of Medicine, 2024) confirms that bromelain supports protein digestion both as a standalone enzyme and synergistically with pancreatic enzymes.
* Immunomodulatory Effects
Bromelain modulates immune cell activation and migration, enhancing innate immune responses while demonstrating anti-allergic properties in animal models. Its Nrf2 antioxidant pathway activation contributes to cellular protection against oxidative stress. These properties make pineapple extract a relevant ingredient for immune support and allergy-related formulations.
* Cardiovascular & Metabolic Support
Emerging research indicates that bromelain may support cardiovascular health through antihyperlipidemic effects - reducing LDL cholesterol and triglyceride levels in animal models - and through inhibition of platelet aggregation. The Frontiers in Nutrition 2026 review also highlights potential antidiabetic effects, with bromelain showing alpha-glucosidase inhibitory activity relevant to blood sugar management.
* Sports Recovery & Muscle Soreness
Bromelain's anti-inflammatory and proteolytic properties have attracted significant interest in the sports nutrition market. Several studies have examined its role in reducing delayed-onset muscle soreness (DOMS) and accelerating recovery after intense exercise - a compelling positioning for sports supplement formulators.
* Antioxidant Activity
The polyphenol and Vitamin C content of whole-fruit pineapple extract contributes meaningful antioxidant activity. Combined with bromelain's Nrf2 pathway activation, pineapple extract offers a multi-mechanism antioxidant profile relevant for anti-aging, skin health, and general wellness formulations.
* Antimicrobial & Antiulcer Properties
Preclinical research has demonstrated bromelain's activity against H. pylori and other gastrointestinal pathogens, as well as protective effects on gastric mucosa. These findings open formulation opportunities in gut health and digestive wellness categories.
Regulatory note for formulators: Most bromelain clinical evidence is derived from in vitro, animal, and small-scale human studies. We recommend working with your regulatory affairs team to ensure finished product claims comply with FDA, EFSA, or Health Canada requirements. The Frontiers in Nutrition 2026 review explicitly notes that "further well-designed clinical studies are required to establish optimal dosing, long-term safety, and clinical efficacy."
Application Scenarios & Formulation Guidance
Pineapple Extract Powder is one of the most versatile botanical ingredients in the nutraceutical toolkit. Here's where leading brands are deploying it:
* Digestive Health Supplements
Enzyme Blends: Bromelain is a cornerstone ingredient in digestive enzyme complexes, typically combined with amylase, lipase, lactase, and papain. Inclusion rates of 250–500 mg per serving (standardized to ≥2,400 GDU/g) are common in clinical-strength formulations. Our high-activity stem extract is the preferred grade for this application.
Gut Health Capsules: Pineapple extract pairs naturally with probiotics, prebiotics, and other digestive botanicals (ginger, papaya, fennel) in comprehensive gut health formulations.
* Sports Nutrition & Recovery
Post-Workout Capsules/Tablets: Bromelain at 500–1,000 mg per serving is increasingly featured in recovery formulations alongside tart cherry, turmeric, and magnesium. The anti-inflammatory mechanism is distinct from NSAIDs, making it attractive for consumers seeking natural recovery support.
Protein Powders: At low inclusion rates (0.5–1%), pineapple extract can serve a dual function - contributing to protein digestion while adding a natural tropical flavor note to plant-based protein formulas.
* Immune Support Formulations
Immune Complex Capsules: Pineapple extract pairs well with Vitamin C, zinc, elderberry, and echinacea in immune support stacks. The bromelain's immunomodulatory activity complements the antioxidant profile of co-ingredients.
* Functional Foods & Beverages
Functional Beverages: The whole-fruit extract grade dissolves cleanly in water-based beverages. Its natural flavor profile is compatible with tropical, citrus, and "wellness shot" flavor systems. Suitable for RTD beverages, effervescent tablets, and powder sachets.
Functional Gummies: Pineapple extract is a natural fit for gummy formats - its flavor, color, and functional profile align perfectly with the category. Note: gummy manufacturing temperatures must be managed carefully to preserve bromelain activity; consult our technical team for formulation guidance.
Baked Goods & Snacks: At low inclusion rates, pineapple extract contributes antioxidant and flavor value to functional snack bars, granola, and baked goods. Note that baking temperatures above 65°C will denature bromelain - for heat-processed applications, the polyphenol and Vitamin C fractions remain active.
* Cosmeceuticals & Topical Applications
Bromelain's proteolytic activity has found application in topical exfoliation and wound-healing products. Pineapple extract is increasingly used in "beauty from within" ingestible skincare formulations targeting skin texture, collagen support, and anti-aging positioning.
* Pharmaceutical & Clinical Nutrition
Bromelain has a documented history of use as an adjunct in post-surgical recovery, particularly in oral and orthopedic surgery contexts. Clinical nutrition manufacturers use standardized bromelain extract in medical-grade formulations targeting inflammation management and tissue repair.
Packaging & Logistics
Standard Packaging Options:
|
Format |
Net Weight |
Suitable For |
|
Double-layer PE bag in fiber drum |
25 kg |
Standard bulk commercial orders |
|
Double-layer PE bag in cardboard drum |
1 kg / 5 kg |
Sample orders, small-batch production |
|
Vacuum-sealed foil bag |
1 kg |
High-activity enzyme grades (moisture-sensitive) |
|
Custom packaging |
Per request |
Private label, co-packing |
Labeling: Each container is labeled with product name, grade/specification, batch number, manufacturing date, expiry date, net weight, storage conditions, bromelain activity (GDU/g), and country of origin
Documentation provided: COA, MSDS, Specification Sheet, Phytosanitary Certificate, Certificate of Origin, Allergen Declaration, and HS code documentation
Lead time: Standard orders (25–500 kg): 7–14 business days after order confirmation
MOQ: 1 kg (sample); 25 kg (commercial)
Shipping: EXW Chongqing / FOB Shanghai / CIF available. Air, sea, and express courier options for North America, Europe, Australia, and Southeast Asia
Storage & cold chain: Standard grades require no cold chain. High-activity enzyme grades (≥2,400 GDU/g) should be shipped with temperature monitoring; we provide ice-pack packaging for air freight upon request

JOYWIN Manufacturing Facilities
JOYWIN founded in 2013 is an innovation-driven biotechnology company. we provide the manufacture of plant extracts, plant proteases, and customized products. We believe Good produce= Integrity+ Technology+Quality Control.

JOYWIN Exhibition Show
JOYWIN attend international exhibitions, including CPhI, FIC, API, Vitafoods, SupplesideWest.

FAQ
Q: What's the difference between GDU/g and MCU/g for bromelain activity? Both are measures of proteolytic enzyme activity, but they use different substrates. GDU (Gelatin Digesting Units) measures activity against gelatin; MCU (Milk Clotting Units) measures activity against casein in milk. GDU is the more commonly used standard in the dietary supplement industry. As a rough conversion: 1 GDU ≈ 1.5 MCU. Always specify which unit system you require when requesting a quotation.
Q: Will bromelain activity be preserved in my finished product? This depends heavily on your manufacturing process. Bromelain is stable up to approximately 65°C and between pH 4–9, but is irreversibly denatured above 65°C. It is also sensitive to prolonged exposure to moisture. For capsule and tablet applications, activity is well-preserved. For gummies, beverages, and baked goods, consult our technical team for formulation-specific guidance.
Q: Is pineapple extract powder suitable for allergen-sensitive consumers? Pineapple is not among the major allergens listed under FDA, EU, or Health Canada regulations. However, some individuals with latex allergy may exhibit cross-reactivity to bromelain (latex-fruit syndrome). We recommend including an appropriate allergen advisory on finished product labels and consulting your regulatory team.
Q: What is the recommended dosage for supplement formulations? Published research has used bromelain doses ranging from 200–2,000 mg per day depending on the application. For digestive enzyme support, 250–500 mg per serving (standardized to ≥2,400 GDU/g) is typical. For anti-inflammatory applications, 500–1,000 mg per day has been used in clinical studies. We recommend consulting your clinical and regulatory affairs team for specific dosage claims.
Q: Can I get an organic-certified version? Yes. We can source NOP (USDA Organic) or EU Organic certified raw material. Please specify this requirement at the inquiry stage, as lead times and pricing differ from conventional grades.
Q: How should I store pineapple extract powder to maintain bromelain activity? Store in original sealed packaging below 25°C, away from direct light, heat, and moisture. For high-activity enzyme grades, refrigerated storage (2–8°C) is recommended for long-term inventory. Once opened, reseal immediately and use within 3 months.
Q: Do you offer stability data for bromelain activity over the 24-month shelf life? Yes. We conduct real-time and accelerated stability testing per ICH Q1A guidelines, with bromelain activity measured at 0, 3, 6, 12, 18, and 24 months. Stability data is available upon request for qualified buyers.
Q: What is your minimum order quantity? 1 kg for sample orders; 25 kg for commercial orders. Volume pricing is available for orders above 100 kg.
Q:Where & how can I place an order?
A:You can click the inquiry on Pineapple extract powder or send us an e-mail to contact@joywinworld.com.
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